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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
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Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Infosomes as Inflammatory Mediators: Proteomic Profiling of Proteins Enriched in Inflammatory Extracellular Vesicles

doi: 10.1016/j.mcpro.2026.101511

Figure Lengend Snippet: Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.

Article Snippet: Primary antibodies used for immunoblotting included High Mobility Group Box 1 (HMGB1) (Abcam Limited, Cambridge, UK; Cat. No. ab18256), copine-1 (Novusbio; Centennial, Cat. No. NBP1-32194), TUBA4a (Cell Signaling Technology, Inc.; Cat. No. #3878), Heat Shock Protein Family A Member 8 (HSPA8) (Santa Cruz Biotechnology Cat. No. SC-7298), TSG101 (Abcam Limited; Cat. No. ab125011), GAPDH (Cell Signaling Technology, Inc.; Cat. No. #2118), NLRP3 (AdipoGen Life Sciences Cat. No. AG-20B-0014-C100), IL-1β (Cell Signaling Technology, Inc.; Cat. No. #12703 or #12426), cleaved IL-1β (Cell Signaling Technology, Inc.; Cat. No. #83186 or #52718), Caspase-1 (Cell Signaling Technology, Inc.; Cat. No. #2225 or AdipoGen Life Sciences; Cat. No. AG-20B-0042–100), ASC (Santa Cruz Biotechnology, Cat. No. SC-514414 or Cell Signaling Technology, Inc.; Cat. No. #67824), and β-Actin (Cell Signaling Technology, Inc.; Cat. No. #3700).

Techniques: Marker, Quantitative Proteomics, Expressing, Western Blot