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Journal: Molecular & Cellular Proteomics : MCP
Article Title: Infosomes as Inflammatory Mediators: Proteomic Profiling of Proteins Enriched in Inflammatory Extracellular Vesicles
doi: 10.1016/j.mcpro.2026.101511
Figure Lengend Snippet: Infosomes are enriched with EV marker proteins. A , volcano plot depicting the differential expression of proteins between infosomes (LN) and conventional EVs (Con). Proteins showing more than twofold upregulation in infosomes are marked in red ( n = 614; p -value <0.05), while those with more than twofold downregulation are indicated in green ( n = 17; p -value <0.05). The accompanying table summarizes the number of proteins showing significant increases or decreases in infosomes relative to conventional EVs. B , cellular component enrichment analysis of proteins upregulated in infosomes. Green boxes indicate junction- and adhesion-related compartments, and blue boxes indicate EV-related compartments. C , expression levels of EV-associated proteins, including High Mobility Group Box 1, copine-1, TUBA4A, Heat Shock Protein Family A Member 8, TSG101 and GAPDH were analyzed by Western blot. WCL and EVs are indicated in the figure. EV, extracellular vesicle; WCL, whole cell lysate.
Article Snippet: Primary antibodies used for immunoblotting included High Mobility Group Box 1 (HMGB1) (Abcam Limited, Cambridge, UK; Cat. No. ab18256), copine-1 (Novusbio; Centennial, Cat. No. NBP1-32194), TUBA4a (Cell Signaling Technology, Inc.; Cat. No. #3878),
Techniques: Marker, Quantitative Proteomics, Expressing, Western Blot